clpV is a key virulence gene during in vivo Pseudomonas plecoglossicida infection

Interaction between bacterial pathogen and aquatic animal host is exceedingly complex, which involves large dynamic changes in gene expression during different stages of the disease. However, research on identifying key virulence genes based on the dynamics of gene expression changes of a one‐sided bacterial pathogen in tissue has not been reported so far across different stages of infectious disease. The clpV for the T6SS of Pseudomonas plecoglossicida was identified for a candidate for key virulence gene based on dynamic changes of gene expression. For the Epinephelus coioides infected using clpV‐RNAi strain, no deaths were observed up to 20 dpi. The spleens, kidneys and livers of all the E. coioides that received clpV‐RNAi strain failed to develop visible nodules at 5–8 dpi, with the swelling gradually disappearing. The burdens of clpV‐RNAi strain in the spleen and blood were greatly reduced at most of the time points after injection, and the burdens of clpV‐RNAi strain in the head kidneys and trunk kidneys also had a sharp reduction from 72 to 120 hpi. This paper provides a new insight into the discovery of key virulence genes of pathogens in infected tissue systems.     

Physicochemical and Structural Properties of Recovered Elastin from Jumbo Squid (Dosidicus gigas) By-Products

Physicochemical and structural properties of soluble jumbo squid (Dosidicus gigas) elastin recovered from skin by-products were evaluated. The molecular weight of isolated elastin was ~40 kDa with an isoelectric point (pI) between 9 and 10. Aspartic, glutamic, arginine, proline, glycine, and lysine amino acids were the most abundant in squid elastin, whereas the hydroxyproline absence, ~0.7% cysteine content, and the calculated 0.35 isoleucine/leucine ratio were used as purity index. Total and reactive sulfhydryl contents were similar (247.0 ± 5.1 vs 242.0 ± 7.5 μmol mg^?1 of protein, P ≥ 0.05) in purified squid elastin but surprisingly higher than previously reported in other elastins. On the other hand, the secondary structures of squid elastin analyzed by Fourier transform infrared spectroscopy (FTIR) were ~45% β-sheets, ~15% α-helices, ~10% β-turns, and ~30% undefined structures. In addition, squid elastin experienced glass transition at 82.01 ± 0.01ºC, denaturation temperature at 110.45 ± 0.64ºC, and aggregation at 197.5 ± 0.23ºC. In conclusion, the prevalence of charged amino acids and pI of squid elastin can facilitate its solubilization in hydrophilic systems, whereas the secondary structure profile and thermostability are desirable features in proteins used for biopolymer designs such as food biofilms or barrier systems.     

广西产糖率下降的原因分析

绪言 时间已经进入到2月中旬，按照大部分糖厂在11月下旬开榨的时间计算，目前各地的榨季生产已经进行了将近三个月的时间。从各地已入榨甘蔗的进度分析，广西大部分糖厂基本上已经接近完成甚至超过了三分之二的榨季生产任务。随着榨季生产的深入，     

Analysis of the influence of land finance on haze pollution: An empirical study based on 269 prefecture-level cities in China

Under the system of political centralization and economic decentralization, the expanding scale of land finance and the increasingly severe environmental pressure have jointly become crucial features of China's urban development. Therefore, it is of great practical significance to study the intrinsic mechanism of land finance on haze pollution for China's economy to achieve kinetic energy transformation and green development. This paper empirically analyzes the impact of land finance on haze pollution using a dynamic spatial Durbin model based on panel data of 269 prefecture-level cities in China from 2004 to 2017. The statistical results show that haze pollution has a significant “snowball effect” and space spillover effect. Land finance has a significant positive effect on haze pollution. Land transfer both by agreement and by bid invitation, auction, and listing have significant positive effect on haze pollution. However, the promoting effect of land transfer by agreement on haze pollution is significantly higher than that of land sale by bid invitation, auction, and listing. Furthermore, regional heterogeneity implies that for cities in the eastern region, land finance is conducive to alleviating haze pollution. In contrast, for cities in the central and western regions, land finance significantly promotes haze pollution.     

川南地区1989—2019年农业干旱危险性特征分析

旨在研究川南地区近30年农业干旱危险性特征。基于川南地区（内江市、自贡市、宜宾市、泸州市）1989—2019年夏季（6—8月）降水数据,组成了降水距平百分率和农业干旱危险性等级之间的模糊隶属函数,通过引入区间平均隶属度,将信息扩散理论与之结合,研究了川南经济区的农业干旱危险性特征。结果显示：轻、中度农业干旱危险性水平最大的都是泸州市,危险性水平分别达21.47%、15.32%;重度农业干旱危险性水平最大的是自贡市,其危险性水平达7.58%;严重农业干旱危险性水平最大的是宜宾市,其危险性水平达7.47%。     

断奶对仔猪肠道屏障的影响及营养调控

在集约化养殖中,3~4周龄早期断奶是仔猪生命周期中一个非常紧张的时期,此时仔猪胃肠道还未发育成熟。肠道屏障由上皮、免疫和肠神经系统组成,这些系统控制上皮屏障的完整性以及肠道功能,包括肠腔营养物质、水和电解质的运输。早期断奶导致肠道通透性增加,出现胃肠功能紊乱的情况,可能对猪只一生产生长期的影响。因此,仔猪断奶饲粮需要正确水平的营养素、营养源和高质量的添加剂,鼓励仔猪快速进食,减轻或消除断奶应激综合症,降低死亡率和发病率。养猪就是养"肠道",合理使用功能性氨基酸、植物化学物质和有机酸等添加剂,能够修复由于断奶应激综合症导致的肠道屏障功能障碍。     

基于有效积温的中国水稻生长模型的构建

有效积温是指作物生长至某一生育阶段所需要积累的有效温度,是反映气象条件对作物生长影响的主要指标,研究有效积温对作物生长过程的影响对提高农业生产效率具有重要意义。该文以有效积温作为气象因子,收集中国气象数据网中的气象数据和已发表的学术论文中的水稻生长数据,建立了描述水稻生长过程的叶面积指数和干物质积累量的普适Logistic模型,并研究了水稻最大叶面积指数与最大干物质积累量、收获指数(作物经济产量与生物产量的比值)及降水量之间的关系。结果表明:有效积温为1000℃左右时,水稻叶面积指数最大,且此时干物质增长速率最大;水稻最大叶面积指数与最大干物质积累量之间表现为线性关系;最大叶面积指数和收获指数、降水量之间为二次抛物线关系,当降水量为670.5 mm时,最大叶面积指数为7.93,对应的水稻收获指数达到最大值0.50。该研究对于构建其他作物的生长模型具有一定的参考意义。     

猪瘟病毒衣壳蛋白与宿主丝/苏氨酸蛋白激酶N1相互作用的鉴定

为筛选并鉴定与猪瘟病毒(CSFV)衣壳(C)蛋白相互作用的宿主蛋白,本研究采用酵母双杂交技术以CSFV C蛋白为诱饵从猪外周血单个核细胞(PBMC)c DNA表达文库中筛选与之相互作用的宿主蛋白,共筛选到6种蛋白,分别为ATP5B、SON3、PKN1、PCBP1、RPS20和IQGAP1,根据Gene Ontology分析结果,这些蛋白分别参与细胞的增殖和代谢等过程。本研究选取丝/苏氨酸蛋白激酶N1(PKN1)进行进一步验证,经酵母共转化试验、免疫共沉淀试验和GST pull-down试验证实,宿主PKN1蛋白与CSFV C蛋白之间存在特异性结合。本研究首次证明PKN1与CSFV蛋白之间的相互作用关系,为进一步研究PKN1蛋白在CSFV感染过程中发挥的作用奠定了基础。     

日本环境保全型耕地农业系统对中国耕地保护的启示

为解决中国农业长期粗放经营问题,研究中国耕地与生态文明协同保护体系,采用文献研究法和归纳演绎法,分析东亚地区代表国家环境保全型农业下的耕地农业协同体系。研究表明,日本的耕地农业环境保护支付制度、生态补偿市场认证体系、耕地农业法律制度保障,是建立在生态补偿原则上,经过实践检验行之有效的,能够提升市场参与度及农民自主性投入的耕地生态协同保护措施。中国在推广耕地合理利用实践方面做出了不懈努力,但是在建立耕地生态协同保护的休耕制度、因地制宜建立生态补偿体系、加快推进耕地生态协同保护专项基金的设立等方面还需进一步坚持和完善。